Gene detail

Detail information of GWHGAAEX016075/evm.TU.scaffold_72.112

Functional Annotation

Database	Orthologs	Annotation
COG	gi\|283778000\|ref\|YP_003368755.1\|	glutamate synthase (NADH) [Pirellula staleyi DSM 6068]
KOG	KOG0399	Glutamate synthase
nr	gi\|672157468\|ref\|XP_008798443.1\|	PREDICTED: glutamate synthase 1 [NADH], chloroplastic [Phoenix dactylifera]
Swissprot	tr\|Q9LV03\|GLUT1_ARATH	Glutamate synthase 1 [NADH], chloroplastic (Precursor)
trEMBL	tr\|A0A067JE60\|A0A067JE60_JATCU	Uncharacterized protein {ECO:0000313\|EMBL:KDP22151.1}
TAIR	AT5G53460	NADH-dependent glutamate synthase 1

Location And Transcript Sequence

Scaffold	Start	End	Strand
GWHAAEX00000071	4133895	4148286	+

Transcript Sequence
>GWHTAAEX016075 ATGGCAGTAGTGCCAAGCACGACCCTTAAGCTTCAGAATGAGTCCGTGGCCATCCATTCAGTGTTTGCAAACAATAAGAAGGTTAGTCGTATCGGGGGCCGAGCCGCGAGATTCTCATTACCATGTCAGTCCCATCGGGTTTCTCTGGAAAATAAATTCCTCGGATCGAAGATGTGCCCTTCGGAGCGAGTTCAGCTATGGTGGACTGATGGGCCTGGGAGATCCCCTAAGCTCAGGGTCGTATCGGCAGCCAAGTCACTGTCTCAGGTACCGGAGAAGCCACTAGGTCTATACGACTCATCTTTTGATAAGGATTCTTGCGGTGTAGGCTTTGTTGCAGAGCTTTCCAGGGAAAACAGAAGGGAGACAGTTGCTGATGCCTTGGAGATGTTAATTAGGATGTCTCACAGGGGAGCATGCGGTTGTGAGGTCAATACAGGCGATGGGGCAGGCATGCTTGTGGCTCTTCCGCATGATTTTTTCAAGGAGGTGACTAAAGGGCTTGGATTTGATTTGCCCTCACCCGGGCAATATGCGGTCGGCATGTTTTTCTTGCCCACAGATCCAAGCCGTCGCAAGGAAAGCAAGCATGTATTTTCCCAGGCTGCAATGTCATTGGGACATGAAGTTATTGGCTGGCGACATGTACCTACAGATAATTCAGATTTAGGAAAGTCTGCTCTTGAGACAGAACCAGTCATTGAACAAGTGTTCCTGAAACCAAGTTTGAGGTCAAGTGTGGATTTTGAACAGCAGATGTACATCTTGAGAAGGGTTTCTATGGTAGCTATTCGAGCTGCTTTAGCCCTCCCGCATGGTGGAGTGAAGGACTTCTACATATGCTCCTTATCTTCAAGGACAGTGGTATACAAAGGACAACTGAAGCCAATTCAGTTAAAAAACTATTTTTATGCAGATCTTGGCGATGAAAGGTTTACAAGTTACATGGCCATGATTCATTCTCGATTTTCCACCAATACCTTTCCTAGCTGGGATCGTGCTCAACCCATGCGCATTTTAGGACATAATGGAGAAATTAATACACTCAAAGGGAATGTAAATTGGATGAAGGCACGTGAGGGTCTCTTGAAGTGCAAGAATTTGAAGTTATCGAAGGATGAAATGAAGAAACTTTTACCCATTGTGGATGTTAGCTCATCAGATTCAGGTGCTTTTGATGGTGTCCTTGAGCTTCTGGTGAGAGCTGGAAGAGATCTTCCTGAAGCTGTCATGATGATGATACCTGAGGCTTGGCAAAATGATCATAACATGGACCCTGAAAGGAAGGCACTCTATGAATATTTTTCAGCACTCATGGAGCCTTGGGATGGACCTGCTTTAGTATCATTTACGGATGGCCGCTACCTTGGTGCAACATTGGATCGTAATGGTTTGAGGCCCGGTCGCTTCTATATTACTCATAGTGGACGTGTAATTATGGCAAGTGAAGTTGGTGTTGTTGACATTCATCCGGAAGATGTTTCCAGGAAAGGAAGGCTCAATCCAGGAATGATGCTATTAGTGGATTTTGAAAAGCACATTGTTGTTGATGATGAAGCATTGAAGAAGCAATATTCATTAGCTCAGCCTTATGGAGAGTGGCTGAAGAAACAGAAGATTACATTGGATGAGGTTGTTGATTCTGTGCCTGAATCAGATAGACTGGCTCCAAGTGTATTTGGAACCAACCCAACTGATGGTTTGGCTGATACTGTTGAAAATATGGGCATGCATGGTCTCTTAGCTCCACTCAAGGCCTTTGGGTATACAGTGGAATCTCTGGAAATGTTGTTACTTCCAATGGCAAAGGATGGCACGGAAGCTCTCGGTTCTATGGGAAATGATGCTCCACTGGCTGTGATGTCAGGCAGGGAGAAACTTACATTTGAGTATTTCAAACAAATGTTTGCACAAGTCACAAACCCTCCAATTGATCCCATTCGTGAAAAGATCGTTACTTCAATGGAATGTATGATTGGTCCTGAAGGTGATCTAACTGAAACTACCGAGGGACAATGCCATCGGCTCACCCTAAAAGGGCCTTTTCTCTCCATTGCGGAAATGGAAGCCATAAAAAAGATGAATTACAAAGGTTGGCGCAGTAAGATACTCAACCTGACTTATCCTAAAAAGCTAGGCCGGAAAGGCTTGGAGGAGACATTGAATAGGATTTGTTCTGAAGCTCGAACTGCTATCTATGAAGGCTACACAACTCTTGTACTATCTGACAGAGGTTTTTCATCGGATTATGTTGCGGTAAGTTCTCTCCTGGCAGTTGGTGCTGTGCATCAGCATCTAGTCTCAACTCTTGAGCGTACTAGGGTGGGGTTGATTGTTGAATCAGCTGAACCCAGGGAAGTTCATCACTTCTGTACACTGGTTGGATTTGGTGCAGATGCTATATGCCCATATCTGGCAATTGAAGCTGTTTGGCGATTACAAATTGATGGAAAGATTCCTCCTAAAGGAAATGGTGAGTTCCACTCGCATGATGAACTCGTCCAGAAGTACTTCAATGCATGCAATGACGGTGTGATGAAAGTTCTTGCTAAGATGGGCATATCTACCCTAGCCTCATACAAAGGTGCACAGATATTTGAAGCCCTTGGACTTTCCACCAAGGTCGTCGACAAGTGTTTTAAAGGCACGCCAAGCAGAGTTGAGGGAGCTACTTTTGAAATGCTAGCCGGGGATGCACTCCGCCTCCATGACATGGCATTTCCCAAACGGGCATTGCCCCCCAGCAGTGCAGATGCAGTGGCGTTACCTAATCCAGGCGACTATCATTGGAGAAAAGATGGTGAGGTGCACCTGAATGATCCTCTCGCAATTGCAAAATTGCAAGAAGCAGCTAGACAAAATAGTGTGTCAGCATACAAAGAGTACTCTAAGCTTATTCAGGAACTAAATAAAGGTTGCAATTTACGAGGGTTGCTCAAGTTCAAGGATGTTCAAGGGAAAATTCCCTTGGATGAAGTAGAACCAGCTTCTGAGATTGTGAAGCGATTCTGTACAGGGGCTATGAGTTATGGTTCTATTTCTTTGGAAACTCATACTACTCTTGCAATTGCCATGAACAAACTTGGTGGAAAATCAAATACAGGTGAGGGAGGAGAACAACCTTCACGAATGGAGCCTCTACCAGATGGATCCAAGAATCCGAAAAGGAGTGCGATCAAGCAAGTTGCAAGTGGAAGGTTTGGGGTTTCAAGCTACTATCTCACCAATGCTGATGAACTTCAAATAAAGATGGCTCAGATGCTGCTGAATCTACAAAGCACCTACGTGTGTCTTTGTATCTAA

Network for GWHGAAEX016075

Network category	Network	Top300 PCC genelist
Coexpression Positive	Network	Top300 PCC genelist
Coexpression Negative	Network	Top300 PCC genelist

Functional Module

Module ID	Function Annotation
Module4

Protein Structure

Pfam accession	Pfam name	Alignment start	Alignment end	E-value
PF00310.20	GATase_2	107	534	7.00E-182
PF04898.13	Glu_syn_central	584	872	4.50E-111
PF01645.16	Glu_synthase	938	1086	2.20E-45

Protein Sequence

>GWHPAAEX016075
MAVVPSTTLKLQNESVAIHSVFANNKKVSRIGGRAARFSLPCQSHRVSLENKFLGSKMCPSERVQLWWTDGPGRSPKLRVVSAAKSLSQVPEKPLGLYDSSFDKDSCGVGFVAELSRENRRETVADALEMLIRMSHRGACGCEVNTGDGAGMLVALPHDFFKEVTKGLGFDLPSPGQYAVGMFFLPTDPSRRKESKHVFSQAAMSLGHEVIGWRHVPTDNSDLGKSALETEPVIEQVFLKPSLRSSVDFEQQMYILRRVSMVAIRAALALPHGGVKDFYICSLSSRTVVYKGQLKPIQLKNYFYADLGDERFTSYMAMIHSRFSTNTFPSWDRAQPMRILGHNGEINTLKGNVNWMKAREGLLKCKNLKLSKDEMKKLLPIVDVSSSDSGAFDGVLELLVRAGRDLPEAVMMMIPEAWQNDHNMDPERKALYEYFSALMEPWDGPALVSFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDIHPEDVSRKGRLNPGMMLLVDFEKHIVVDDEALKKQYSLAQPYGEWLKKQKITLDEVVDSVPESDRLAPSVFGTNPTDGLADTVENMGMHGLLAPLKAFGYTVESLEMLLLPMAKDGTEALGSMGNDAPLAVMSGREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEGQCHRLTLKGPFLSIAEMEAIKKMNYKGWRSKILNLTYPKKLGRKGLEETLNRICSEARTAIYEGYTTLVLSDRGFSSDYVAVSSLLAVGAVHQHLVSTLERTRVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAVWRLQIDGKIPPKGNGEFHSHDELVQKYFNACNDGVMKVLAKMGISTLASYKGAQIFEALGLSTKVVDKCFKGTPSRVEGATFEMLAGDALRLHDMAFPKRALPPSSADAVALPNPGDYHWRKDGEVHLNDPLAIAKLQEAARQNSVSAYKEYSKLIQELNKGCNLRGLLKFKDVQGKIPLDEVEPASEIVKRFCTGAMSYGSISLETHTTLAIAMNKLGGKSNTGEGGEQPSRMEPLPDGSKNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQMLLNLQSTYVCLCI

Gene family

Gene family	subfamily
--	--

KEGG pathway

KO	Enzyme	pathway	mapID
K00264	GLT1	Metabolic pathways	map01100
		Biosynthesis of secondary metabolites	map01110
		Microbial metabolism in diverse environments	map01120
		Biosynthesis of antibiotics	map01130
		Biosynthesis of amino acids	map01230
		Nitrogen metabolism	map00910
		Pyrimidine metabolism	map00240

Gene Ontology

GO term	Ontology	Name
GO:0055114	Biological Process	oxidation-reduction process
GO:0006537	Biological Process	glutamate biosynthetic process
GO:0006807	Biological Process	nitrogen compound metabolic process
GO:0016638	Molecular Function	oxidoreductase activity, acting on the CH-NH2 group of donors
GO:0015930	Molecular Function	glutamate synthase activity
GO:0003824	Molecular Function	catalytic activity

Expression pattern

SRP	Sample	Description	FPKM
SRP064423	SRR2545914	vegetative propagation corms	125.840
SRP064423	SRR2545915	Juvenile tuber	127.820
SRP108465	SRR5643513	five period of Gastrodia elata	67.110
	SRR5643514	five period of Gastrodia elata	76.910
	SRR5643515	five period of Gastrodia elata	103.300
	SRR5643524	five period of Gastrodia elata	107.710
	SRR5643525	five period of Gastrodia elata	104.020
	SRR5643528	five period of Gastrodia elata	277.960
	SRR5643658	five period of Gastrodia elata	131.740
	SRR5643659	five period of Gastrodia elata	88.250
	SRR5643660	five period of Gastrodia elata	90.210
	SRR5643661	five period of Gastrodia elata	75.150
	SRR5643662	five period of Gastrodia elata	70.740
	SRR5643663	five period of Gastrodia elata	73.790
	SRR5643664	five period of Gastrodia elata	66.400
SRP118053	SRR6049654	Rhizomagastrodiae_C368-T07_good_1	61.440
	SRR6049655	Rhizomagastrodiae_C368-T10_good_1	26.950
	SRR6049656	Rhizomagastrodiae_C368-T07_good_1	48.740
	SRR6049657	Rhizomagastrodiae_C368-T07_good_1	45.480
	SRR6049658	Rhizomagastrodiae_C368-T04_good_1	47.610
	SRR6049659	Rhizomagastrodiae_C368-T04_good_1	45.370
	SRR6049660	Rhizomagastrodiae_C368-T01_good_1	48.450
	SRR6049661	Rhizomagastrodiae_C368-T04_good_1	44.160
	SRR6049662	Rhizomagastrodiae_C368-T01_good_1	93.190
	SRR6049663	Rhizomagastrodiae_C368-T01_good_1	85.200
	SRR6049664	Rhizomagastrodiae_C368-T10_good_1	50.910
	SRR6049665	Rhizomagastrodiae_C368-T10_good_1	50.910
SRP279888	Gastro	Gastrodia elata tuber	63.92
	Juven	Juvenile tuber	317.85
	Mother_Gastro	Mother tuber of Gastrodia elata	358.39
	Mother_Juven	Mother tuber of Juvenile	1,174.06